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Filler enchanced natural rubber is widely used in transport and national defence and carbon black has given much contribution on the mechanical properties of rubber composites.Because of complex of network structures and limitations of research methods, how the influence of some key factors of carbon black on the mechanisms of bound rubber, such as diameters, structures and shapes, is under debate, and the three dimensional structures of carbon blacks distributing in the rubber matrix is not clear.Aim at the challenge, this study develops micro-mechanical measurement set up which can be used in in-situ measurement with synchrotron X-ray 3D imaging techniques.Taking carbon black enhanced natural rubber as example, by developing in-situ micro-probe technique, we will accomplish multi-scale structure characterization with time and spatial resolution accompanying with mechanical properties measurement.and the proper principles of carbon black enchancing the natural rubber together with the relationship between the Payne effect of filled natural rubber and three dimensional structures of carbon black in rubber matrix will be given.We aim at establishing synchrotron-based method for the study of mesoscopic mechanics in polymer and providing more useful help for the users from polymer materials and other research filed as well as give evidences for designing and producing the natural rubber composites with special mechanical properties.