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In this study,IMPI in Eimeria tenella was predicted to be a membrane protein,consistent with the transmembrane domain prediction result.To evaluate immunogenicity of IMPI in Eimeria tenella,a chimeric subunit vaccine consisting of E.tenella IMP 1 (EtIMP 1) and a molecular adjuvant (a truncated flagellin,FliC) was constructed and over:expressed in E.coli and its efficacy against E.tenella infection was evaluated.ThreE -week:old AA broiler chickens were vaccinated with the recombinant EtIMP 1:truncated FliC without adjuvant or EtIMP1 with Freund s Complete Adjuvant.Immunization of chickens with the recombinant EtIMP 1: truncatedFliC fusion protein resulted in stronger cellular immune responses than immunization with only recombinant EtIMP 1 with adjuvant.The clinical effect of the EtIMP 1:truncated FliC without adjuvant was also greater than that of the EtIMP 1 with adjuvant,which was evidenced by the differences between the two groups in body weight gain,oocyst output and caeeal lesions of E.tenella:challenged chickens.The results suggested that the EtIMP 1:?agellin fusion protein can be used as an effective immunogen in the development ofsubunit vaccines against Eimeria infection.This is the first demonstration of antigen:specific protective immunity against avian coccidiosis using a recombinant Flagellin as an apicomplexan parasite vaccine adjuvant in chickens.