Purpose: To evaluate the value of length and offset varied saturation (LOVARS) and amide proton transfer (APT) in the diagnosis of gliomas,. Methods: 16 patients with 12 high-grade and 4 low-grade gliomas confirmed pathologically were recruited in this study. All MR imaging was performed on a 3.0 T MR scanner with a 12-channel head coil. Axial and sagittal T1-weighted and T2-weighted images were acquired for morphological analysis. APT and LOVARS imaging were performed using a prototype 3D GRE CEST sequence with TR/TE of 1620/2.87 ms, FA of 12, slice thickness of 5 mm, FOV of 256′256 mm2, and matrix of 128′128. ROIs were placed in the area of solid tumor component to measure the singles intensity in APT.Results: Figure 1 showed the example cases of conventional MRI, APT and LOVARS phase images in a low-grade and a highgrade glioma. In the the low-grade glioma, APT did not show significant differences between tumor area and background tissues (P >0.05), but showed high single intensity in the tumor area in high-grade glioma (P <0.001). The signal intensity in high-grade glioma is higher than in low-grade glioma in APT images (P <0.01). In LOVARS phase images, both low-grade and high-grade showed higher signals in tumor area than the background tissues (P <0.05), and there was no significant difference between the signal intensities in low-grade and high-grade tumors. Conclusion: APT images is useful in distinguishing low-grade and high-grade glioma, while LOVARS phase images shows better contrast between glioma and peripheral tissue, and the internal heterogeneous components of the glioma.