@@Gold nanoparticles (AuNPs) are becoming increasingly attractive for many scientific fields because of their long-term stability, high surface area-to-volume ratio, and ease of chemical modification. Since the demonstration that thiol (SH) or amino (NH2) groups can bind to gold to form self-assembled monolayers (SAMs), use of these thin films has become ubiquitous in biochemical, materials science, and other applications. However, only a small amount of research has been devoted to the application of AuNPs for separation science. Gold based stationary phases have the great potential to become promising substitutes to silica based materials as stationary phase for capillary electrochromatography (pCEC) and μHPLC due to their stability at high pH, enough rigidity, and ease of chemical modification. However, SAM modified pure gold micro-spheres were, up till now, not investigated as an alternative packing material to silica for column packing in either pCEC or μHPLC. In this presentation, we will report our results from three projects associated with gold based stationary phases utilized in pCEC and μHPLC.