【摘 要】
：
我们曾观察到,大鼠鞘内注射高剂量ryanodine耗竭胞内钙库,可以阻断强直刺激坐骨神经诱发的脊髓背角场电位C反应的长时程增强(LTP).在脊髓薄片标本和在体脊髓上,本工作进一步研究ryanodine受体在脊髓痛敏神经元突触可塑性变化中的作用.(1)鞘内注射ryanodine(1,0.1,0.01和0.001mM,10μL)前12分钟给予ryanodine受体的内源性激动剂cADPR(0.1mM,
【机 构】
：
复旦大学神经生物学研究所,上海200032
【出 处】
：
中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
论文部分内容阅读
我们曾观察到,大鼠鞘内注射高剂量ryanodine耗竭胞内钙库,可以阻断强直刺激坐骨神经诱发的脊髓背角场电位C反应的长时程增强(LTP).在脊髓薄片标本和在体脊髓上,本工作进一步研究ryanodine受体在脊髓痛敏神经元突触可塑性变化中的作用.(1)鞘内注射ryanodine(1,0.1,0.01和0.001mM,10μL)前12分钟给予ryanodine受体的内源性激动剂cADPR(0.1mM,10 μL),C纤维诱发脊髓场电位的基础反应不受影响,但可以阻断Ryanodine对脊髓场电位C反应LTP的抑制作用,提示Ryanodine敏感的胞内钙库参与脊髓背角C反应LTP的形成.(2)在带背根(长度：0.6-1.2 mm)的离体脊髓切片(厚度：500 μm)上,从背根给强直电刺激可诱导出场电位C反应的LTP.在灌流液中加入ryanodine(7 μm)对基础C反应没有明显的影响,但是ryanodine预处理抑制了C反应LTP的诱导.与在体记录的结果一致,灌流ryanodine前10分钟,在人工脑脊液中加入ryanodine受体的内源性激动剂cADPR,同样翻转了ryanodine对LTP的抑制效应.本研究进一步证明了ryanodine受体在脊髓LTP诱导中的重要作用.
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