The victims radiated by high-dose in a short time are often accompanied by symptoms of systemic reaction,such as systemic inflammation,nausea,slower growth and so on.X-ray exposure produce persistent difficulties in memory through an unknown mechanism.Insulin-like growth factor 1(IGF-1),a protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development.Here,in order to study the relationship between IGF-1expression pattern and X-ray exposure in brain of Tibet Minipigs,not castrated male Tibetan minipig were chose as animal model,and exposed to the total body X-ray radiation at 0 and 10 Gy.The expression levels of IGF-1 was quantified in brain tissues of Tibetan minipig by qRT-PCR.Protein levels of IGF-1 and related signalling pathways were determined by western blot analysis.U251 cells was used for vitro experiments.Lentiviral vector had been successfully constructed for transfecting the cell line of IGF-1 over-expression and low-expression.The cell proliferation was evaluated by MTT assay,and the apoptosis was evaluated by flow-cytometric.We found that the proliferation of IGF-1 overexpression cell line was higher than the control group after X-ray irradiation (P<0.05).IGF-1,a molecule implicated in suppressing apoptosis,and partially counteracted the effects of high doses of X-ray exposure on brain cell proliferation.