【摘 要】
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Since the concept of aptamer was proposed in 1990 by Tuerk and Ellington,many new methods for aptamer selection and application have been deveoped.Capillary
【机 构】
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SchoolofLifeScience,BeijingInstituteofTechnology,Beijing,100081,China
【出 处】
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第九届全国微全分析系统学术会议、第四届全国微纳尺度生物分离分析学术会议、2014国际微流控芯片与微纳尺度生物分离分析学术
论文部分内容阅读
Since the concept of aptamer was proposed in 1990 by Tuerk and Ellington,many new methods for aptamer selection and application have been deveoped.Capillary electrophoresis(CE)is one of the most powerful methods for aptamers sieving(known as CE-SELEX),which has the advantages of fast,high resolution,low sample consumption and smart separation modes of capillary zone electrophoresis(CZE),affinity capillary electrophoresis(ACE),low pH mode,etc.Moreover,the binding of target and synthetic single stranded DNA(ss-DNA)occurs in free solution,which eliminates the biases caused by stationary support and linker.Some proteins aptamers have been successfully obtained based on CE,in which the selection efficiency is greatly improved.
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