Centrosome is not only the center of microtubule organization,but is also an important part of regulator of cell proliferation.CrpF46 (centrosome rehted protein F46) is a novel protein discovered by our group a few years ago.In previous study,we found CrpF46 localized on the centrosome in a cell cycle dependent manner,and its aberrant expression could cause disorganized centrosome proliferation,resulting in cell apoptosis.However,the mechanism by which CrpF46 regulates cell proliferation has not been known yet.In this study,we performed a yeast two hybrid screen to identify proteins directly interacting with CrpF46.PCNA (proliferating cell nuclear antigen) was one of the proteins identified through the screen,and subsequent researches revealed that PCNA interacted with CrpF46 in HeLa cells as well.We also demonstrated that the first coiled-coil domain of CrpF46 responds to binding PCNA.