目的:观察血管内皮Jagged1下调对血小板衍生的生长因子(platelet derived growth factor,PDGF)诱导平滑肌细胞增殖迁移的影响。方法:分离培养大鼠主动脉内皮和平滑肌细胞,将内皮接种于下室、平滑肌接种于上室建立细胞共培养体系,根据内皮是否行Jagged1小RNA干扰分为对照组、空载体组和Jagged1小RNA干扰组。用Western blot检测内皮 OBJECTIVE: To observe the effect of Jagged1 vascular endothelial reduction on the proliferation and migration of smooth muscle cells induced by platelet derived growth factor (PDGF). Methods: The aortic endothelial cells and smooth muscle cells of rats were isolated and cultured. The endothelium was inoculated into the lower chamber and the smooth muscle was inoculated into the upper chamber to establish a cell coculturing system. According to whether Jagged1 small interfering RNA was involved in the endothelium, RNA interference group. Endothelial cells were detected by Western blot