【摘 要】
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A sequence-indexed,user-friendly,reverse genetics resource is highly desirable to fully exploit the maize genome sequence.This NSF-PGRP-funded project is generating and sequence-indexing a collection
【机 构】
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Waksman Institute,Rutgers University,Piscataway,NJ 08854
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A sequence-indexed,user-friendly,reverse genetics resource is highly desirable to fully exploit the maize genome sequence.This NSF-PGRP-funded project is generating and sequence-indexing a collection of Ds transposon insertions using a cost-effective method that takes advantage of next-generation sequencing (NGS) technologies.Specifically,the goals for this project are to:(1) Assemble a set of 120 roughly equidistant Ds * launching platforms carrying a GFP marker that allows simple visual selection of element transposition from any region of the genome and,thus,enable researchers to generate regional gene knock-out collections;(2) Sequence-index several thousand Ds* insertion sites from dozens of model platforms by NGS of 3-dimensional DNA pools;and (3) Place all relevant information in our web-searchable database of insertion site sequences (http://acdsinsertions.org) cross-referenced to stocks available from the Maize Genetics Stock Center.
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