Trx-TSP50融合蛋白的克隆表达、纯化及鉴定

来源 :中华医学会第九次全国核医学学术会议 | 被引量 : 0次 | 上传用户:iamformywish
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目的 本实验旨在研究Stmn1和Sept-9在骨髓基质干细胞诱导分化为胆碱能神经元过程中的表达变化情况.方法 采用全骨髓培养法体外分离培养获得Wistar大鼠骨髓基质干细胞,用含10%胎牛血清的DMEM培养基培养,然后使用不含胎牛血清,并添加20 ng/mL bFGF,5 μmol/L RA和20 ng/mL Shh蛋白的DMEM/F 12培养基进行诱导分化,观察骨髓基质干细胞向胆碱能神经元分化的
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