【摘 要】
：
Cherriistry and Molecular Sciences,Wuhan University Wuhan 430072,PR China Foodborne illnesses have always been a serious problem that threats public health,
【机 构】
：
Key Laboratory of Analytical Chemistry for Biology and Medicine(Ministry of Education),College of Ch
【出 处】
：
第九届全国微全分析系统学术会议、第四届全国微纳尺度生物分离分析学术会议、2014国际微流控芯片与微纳尺度生物分离分析学术
论文部分内容阅读
Cherriistry and Molecular Sciences,Wuhan University Wuhan 430072,PR China Foodborne illnesses have always been a serious problem that threats public health,so it is urgent to develop a method that can rapidly and sensitively detect pathogens at low levels in food.Immunomagnetic isolation in microfluidics has emerged as a technique for the separation and enrichment of pathogens.In this paper,we reported a magnetic-controlled microfluidic system [l] which integrated the separation,enrichment and detection of S.typhimurium.The antibody modified magnetic nanospheres synthesized by LBL method [2] can specifically capture the S.typhimurium.Then the magnetically captured S.typhimurium were separated,enriched and detected in a single chip under a fluorescence microscopy based on the principle of sandwich immunoassays.In addition,this method had excellent selectivity and specificity,good reproducibility and strong anti-interference ability.It had been successfully used to detect S.typhimurium synthetic samples(milk,fetal bovine serum).The developed system provides a rapid and sensitive tool to image and quantify pathogens at low levels with large sample volumes in practice.
其他文献
Isolation and release of rare,pure and viable circulating tumor cells(CTCs)provides a significant advance in early cancer detection.However,the low occurren
附子是著名传统中药和川产道地药材，为毛茛科乌头属乌头(AconitumcarmichaeliDebx.)的子根，具有重要的药用价值。 本研究对四川江油、四川布拖、陕西汉中、云南禄劝等附子主
A novel thiol grapheme(TG)functionalized poly[ionic liquid(ViOcIm+Cl-)] boronate affinity monolithic column was prepared in a single step with 4-vinylphenyl
A strategy for simultaneously multiplexed DNA detection by an effective microfluidic droplets manipulation,which is called “assembly-line manipulation of d
榆黄蘑（Pleurotus citrinopileatus），又名金顶侧耳，在分类学上属于真菌门（Eumycophyta），担子菌亚门（Basidiomycotina），层菌纲（Hymenomycetes），伞菌目（Agaricales），侧耳科（Pleurotaceae），侧耳属（Pl
突出重点分类指导中共上海市浦东新区工委组织部党的十四届四中全会以来，我们根据中央和上海市委的要求和部署，始终把开展党员“双学”活动放在实施党的建设新的伟大工程的重要
大粒径硅溶胶具有优异的粘结性能、吸附性能及稳定性能，使其在众多领域中得到广泛的应用。硅溶胶的性能不仅取决于其浓度、粘度等性质，还与胶粒粒径大小和结构密切相关，因此大粒
随着能源的枯竭,新能源的开发已成为社会热点。其中纤维素生物质转化生产燃料乙醇因为其原材料丰富和持续可再生而倍受重视。但其经济性特别是能耗问题是该过程工业化的关键
“为人民盖房，对人民负责，让人民放心”是天津建工集团三建建筑工程有限公司项目经理范玉恕不懈的追求。他主持工程施工３０余万平方米，其中建成的１７项，２０多万平方米建筑，项项优良，两项工程