Nuclear prelamin A recognition factor, Narf, was identified by screening a human skeletal muscle library in a yeast twohybrid interacting screen, which sequence was in agreement with narf from HeLa cells.Narf gene was obtained by PCR and cloned into prokaryotic expression vector pGEX-4T-1, generating the recombinant vector pGEX-Narf.BL(DE3) transfected with pGEX-Narf was inducted 5h by IPTG.A weak predicted 86-kD-band, the recombinant protein, and a 24-kD-degradation-band were showed by SDS-PAGE indicating that degradation of the recombinant protein was occurred, which laid a foundation for further function analysis of Narf.