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The effects of feeding different dosages of a metabolite combination of L.plantarum RS5,RI11,RG14 and RG11 strains (Com3456) on cholesterol reduction in plasma and meat in broiler chickens and the possible mechanism was studied.A total of 504,male Ross broilers were grouped into 7 treatments and offered different diets including a standard corn-soybean based diet (-ve control),a standard cornsoybean based diet + neomycin and oxytetracycline (+ve control) as well as a standard corn-soybean based diet supplemented with 0.1,0.2,0.3 0.4 or 0.5% of Com3456.