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目的建立高效液相色谱法同时测定八味檀香散中丁香酚和甘草酸含量的方法。方法采用Agilent ZORBAX EclipseSB-C18(4.6 mm×150 mm,5μm)色谱柱。流动相为乙腈-5%乙腈(含3%冰醋酸)(27∶73);流速1 mL.min-1,检测波长程序:0～17.0 min为280 nm,17.0～30 min为250 nm。结果丁香酚和甘草酸保留时间分别约为15和21 min,与各自相邻峰的分离度均大于1.5。以峰面积对进样质量浓度(μg.mL-1)线性回归,丁香酚回归方程:Y=0.106 4ρ-1.338,r=0.999 9,线性范围77.76～777.6μg.mL-1。甘草酸回归方程:Y=0.133 9ρ-0.222 0,r=0.999 9,线性范围14.40～144.0μg.mL-1。丁香酚和甘草酸的回收率分别为99.0%和97.3%、RSD分别为1.5%和1.8%。结论本法操作简便,测定结果准确,重复性好,可用于八味檀香散中丁香酚和甘草酸的含量测定。
OBJECTIVE To establish a method for the simultaneous determination of eugenol and glycyrrhizic acid in Bawei Sandalwood by high performance liquid chromatography. Methods Agilent ZORBAX EclipseSB-C18 (4.6 mm × 150 mm, 5 μm) columns were used. The mobile phase was acetonitrile-5% acetonitrile with 3% glacial acetic acid (27:73). The flow rate was 1 mL.min-1. The detection wavelength was 280 nm at 0-17.0 min and 250 nm at 17.0-30 min. Results The retention time of eugenol and glycyrrhizic acid was about 15 and 21 min, respectively, and the resolution of each peak was higher than 1.5. The peak area of the injection mass concentration (μg.mL-1) linear regression, eugenol regression equation: Y = 0.106 4ρ-1.338, r = 0.999 9, the linear range of 77.76 ~ 777.6μg.mL-1. Glycyrrhizic acid regression equation: Y = 0.133 9ρ-0.222 0, r = 0.999 9, the linear range of 14.40 ~ 144.0μg.mL-1. The recoveries of eugenol and glycyrrhizic acid were 99.0% and 97.3%, respectively, with RSDs of 1.5% and 1.8%, respectively. Conclusion The method is simple, accurate and reproducible. It can be used for the determination of eugenol and glycyrrhizic acid in Bawei Sandalwood powder.