Multiple sclerosis (MS) is an autoimmune disease characterized by demyelization, inflammation and neuron degeneration in the central neural system (CNS).At present, immune suppression drugs are usually used for MS treatment with less recovery of damaged neural tissues.Here we investigated the therapeutic effects of iPSC-derived neuron stem cell (NSC) for MS therapy.NSCs differentiated from iPSC were transplanted into the lateral ventricles of Experimental Autoimmune Encephalomyelitis (EAE) mice.After receipt of NSC transplantation, the EAE mice showed reduced area of demyelization, increased oligodendrocyte cell numbers, and improved exercise capacity.Immune staining assay showed less infiltration of T cells in the EAE mice, supporting the therapeutic effects of MS treatment by NSC transplantations.Furthermore, no primed T cells against iPSC-NSCs were detected in the transplanted EAE mice, indicating limited immunogenicity of iPSC-NSCs.These data support the feasibility to use autologous NSC to cure MS disease.