In quantitative shotgun proteomics,advanced mass spectrometry(MS) coupled with diverse stable isotope labeling methods(e.g.,ICAT,iTRAQ,mTRAQ,TMT,and etc.) has been considered as an effective solution in the determination of targeted protein(s) of interest and offers the information of targeted protein-specific metabolic functions in a cell and pathogenically their interconnections with the virulence factors related to diverse human diseases.