Objectives Recent studies have demonstrated that antidepressant effect of N-Methyl-D-aspartate (NMDA) antagonist ketamine activates rapidly the mammalian target of rapamycin (mTOR) pathway and increase synaptic proteins the prefrontal cortex.mTOR is a protein kinase involved in the regulation of translation initiation and protein synthesis required for synaptic plasticity.Recent studies suggest that mTOR activation may be related to the antidepressant action.However,the mTOR signaling underlying antidepressant drugs action has not been well elucidated.The aim of the present study was to find out whether alterations in mTOR signaling could be observed following treatment with tianeptine.Additionally,we investigate whether this drug affect the synaptic proteins and neurite outgrowth via mTOR signaling.