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Highly effective strains and corresponding culture techniques are the bases and core contents in industrial microbiology.Screening and conditions optimization are also regular process in the whole microbiological production process.Traditional microbial screening procedures using flasks are highly labor intensive,take long time and are in poor efficiency.Microfluidics has the potential to solve these problems in microbial screening process and will be widely used in future.However,current research on microfluidic microbial screening system is in its infancy.Lack of microfluidic chips for massively parallel suspension culture of microorganisms and efficient analysis of microbial samples are the bottleneck for the application of microfluidics on microbial screening processes.So,we aim to develop an integrated microfluidic chip system for microbial screening and optimization to meet the demands of high throughput,automation and varied culture conditions in screening process.Here we present a microfluidic chip that integrates 20 parallel culture and analysis units in 2 columns.Four ordinary experimental procedures in microbial screening and conditions optimization involving gradient generation,suspension culture,batch sampling and glucose analysis are serially achieved on the chip.With this chip system,improved strain candidates could efficiently identified,and the optimization process of culture conditions could also be accelerated,which could promote the development of industrial microbiology.