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In the present report,based on a PAMAM derivative(PAMS),a degradable gene delivery system(PAMS/DNA/10NLS)containing nucleus location signal peptide(NLS)was prepared.The agarose gel electrophoresis,particle size and zeta potential of PAMS/DNA/10NLS were similar to those of PAMS/DNA,which proved that NLS did not affect the interaction between PAMS and DNA.