【摘 要】
：
Organic acids are essential to fruit flavor.The vacuolar H+ transporting adenosine triphosphatase(V-ATPase)plays an important role in organic acidtransport and accumulation.However,less is known of V-
【机 构】
：
College of Agriculture & Biotechnology/Zhejiang Provincial Key Laboratory of Horticulture Plant Inte
【出 处】
：
2015 International Conference on Fruit Quality Biology (第二届果
论文部分内容阅读
Organic acids are essential to fruit flavor.The vacuolar H+ transporting adenosine triphosphatase(V-ATPase)plays an important role in organic acidtransport and accumulation.However,less is known of V-ATPase interacting proteins and their relationship with organic acid accumulation.The relationship between V-ATPase and citric acid was investigated,using the citrus tangerine varietiesOrdinary Ponkan(OPK)and an early maturing mutantZaoshu Ponkan(ZPK).Five V-ATPase genes(CitVHA)were predicted as important to citric acid accumulation.Among the genes,CitVHA-c4 was observed,using a yeast two-hybrid screen,to interact at the protein level with an ethylene response factor,CitERF13.This was verified using bimolecular fluorescence complementation assays.A similar interaction was also observed between Arabidopsis AtERF017(a CitERF13 homolog)and AtVHA-c4(a CitVHA-c4 homolog).A synergistic effect on citric acid levels was observed between V-ATPase proteins and interacting ERFs when analyzed using transient over-expression in tobacco and Arabidopsis mutants.Furthermore,the transcript abundance of CitERF13was concomitant with CitVHA-c4.CitERF13 or AtERF017 over-expression leads to significant citric acid accumulation.This accumulationwas abolished in an AtVHA-c4 mutant background.ERF-VHA interactions appear to be involved in citric acid accumulation,which was observed in both citrus and Arabidopsis.
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