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IL-2 is a pleiotropic cytokine produced after antigen activation that plays pivotal roles in the immune response and is an approved drug used for various kinds of diseases such as cancer and dermatitis.In this study, we explored the effects of IL-2 on phenotypic and functional maturation of murine BMDCs.Phenotypic maturation of BMDCs under influence of IL-2 was analyzed by light microscope, flow cytometry(FCM) and functional maturation of BMDCs were confirmed by transmission electron microscopy (TEM), cytochemistry assay, acid phosphatase (ACP) activity, FITC-dextran, bio-assay and enzyme linked immunosorbent assay (ELISA).We elucidated that IL-2 up-regulated the expression of key surface markers such as: CD40, CD80, CD83, CD86 and MHC Ⅱ molecules of BMDCs,down-regulated pinocytosis and phagocytosis activity, induced more production of IL-12 and TNF-α by BMDCs.It is therefore concluded that IL-2 can effectively enhance the maturation of BMDCs.Our results provide direct evidence on using IL-2 as an immune remedy for cancer treatment and a potent adjuvant in preparation of DC-based vaccines.