In this paper, a MIP coating grafted to a trimethylolpropane trimethacrylate (TRIM) core material in a capillary for electrochromatography (CEC) chiral separation of amlodipine was reported.A core monolith was prepared using a solution of 40% (w/w) TRIM by using a mixture of porogen and a polymerization precursor, which was used to generate a stable electroosmotic flow CEC by post-polymerization hydrolyzition to introduce an ionizable functional monomer.Graft polymerization took place on the resultant TRIM monolith with a mixture of (S)-amlodipine /MAA/EDMA.Strong recognition ability (selectivity factor was 6.71) and high column performance (theory plates was 13,988 plates/m) of the template were obtained on the resulting grafted imprinted monolith in CEC mode.The resolution of enatiomers separation achieved on the (S)-amlodipine-imprinted column was up to 13.3.The influence of the CEC conditions on chiral separation, including the composition of the mobile phase, pH value and the operating voltages was also studied.These results suggest that this grafted polymerization technique of allows the imprints to be directly created on the surface of the monolithic material using a minimum amount of template and is a good alternative to prepare CEC-based monolithic MIPs rapidly.