UV rays were used as mutagen in wild type strain of Aspergillus niger for enhanced production of glucose oxidase.After mutagenization and selection,mutant Aspergillus niger strains,resistant to 2-deoxy-D-glucose were obtained.The mutants showed 1.57 and 1.98 fold increase in activities of extra and intra cellular glucose oxidase respectively in comparison with the parental strain.Out of 6 mutants,mutant U-6 was selected as the best producer of glucose oxidase after 36 hours of fermentation using black strap molasses as substrate.