Transient receptor potential (TRP) channels function as cellular sensors to perceive and respond to a variety of environmental stimuli including temperature, pain, pressure and fluid flow.These channels can be divided into seven subfamilies, including TRPV, TRPC, TRPP, and four others.Functional TRP channels are tetrameric complexes consisting of four pore-forming subunits, which could be identical (homotetrameric channels) or different (heterotetrameric channels).In the present study, we found that TRPV4,-C 1, and-P2 associate together to form a physical complex.In function study, this TRPV4-C 1-P2 complex mediates flow-induced Ca2+ influx in HEK293 cells over-expressing TRPV4,-C 1, and-P2 and in rat mesenteric artery endothelial cells (MAECs).