Osteoporosis is characterized by the imbalance of two relatively independent processes-osteoblastogenesis and osteoclastogenesis.Calcineurin (Cn)/nuclear factor of activated T cells (NFAT)(Cn/NFAT) si
目的 中医药在促进骨折愈合方面的功效,虽为临床实践所证实,但很难获得符合现代科学研究规范的临床证据。本研究建立大鼠股骨干骨折模型,从X线表现和病理学方面,验证右归饮是否具有促进骨折愈合作用。方法：采用清洁级雄性Wistar大鼠54只,适应喂养1周后,按随机编号后分别除以3,按余数0、1、2分为三组,A组为对照组,18只；B组为模型组,18只；C组为治疗组,18只。水合氯醛腹腔内注射麻醉成功后,A组
ObjectiveThe present paper describes a comparative characterization of cytotoxic effects of doxycycline and cisplatin on osteosarcoma cells by using impedance sensing techniques.Materials and MethodsT
Background There is conflicting evidence regarding the association between decreased bone mineral density (BMD) and atherosclerosis.To this end,we performed a systematic review and meta-analysis to cl
目的 改良体外分离培养、扩增、鉴定大鼠毛囊干细胞(rHFSCs)的方法,观察其生物学特性,并在特定条件下诱导分化,探讨其成脂成骨分化能力。方法：无菌条件下切取1周龄SD大鼠触须部皮肤,用Dispase酶和Ⅳ型胶原酶混合液消化,显微镜下分离毛囊隆突部,用梯度加液组织块贴壁法培养rHFSCs,细胞融合达80％时消化传代,用Ⅳ型胶原差速贴壁法纯化。
Methods 12 sacral schwannoma cases operated at Zhejiang University,School of Medicine,Second Affiliated Hospital,Department of Orthopaedics from 2012 to 2016 are investigated retrospectively and 11 pa
目的 探讨体外进行骨髓间充质干细胞-软骨细胞-成骨细胞-人羊膜衍生膜(human acellular amniotic membrane,HAAM)共培养,构建软骨组织以优化组织工程软骨的种子细胞源的可行性。方法 分离培养骨髓间充质干细胞,软骨细胞及成骨细胞,并进行鉴定。后将第3代BMSCs与软骨细胞按一定比例(4：1)混合,接种于HAAM上,再加入成骨细胞进行非接触式共培养作为实验组(A组)。以
目的 研究HSP70对骨髓间充质干细胞成骨分化的影响及其相关机制方法：在成骨分化培养基中添加不同浓度热休克蛋白70(HSP70)对人骨髓间充质干细胞(hMSC)进行培养。采用碱性磷酸酶(ALP)活力检测以及茜素红染色了解HSP70在成骨分化早期和晚期的影响。采用PCR及WB技术检测成骨相关标志物的表达变化。最后通过基因芯片技术和通路富集分析研究HSP70促进成骨分化相通路。
HSPA1A,which encodescognate heat shock protein 70,plays important roles in variouscellular metabolic pathways.To investigate its effects on osteogenicdifferentiation of bone marrow mesenchymal stem ce