研究外源性Rad基因重组腺病毒在体外转染乳鼠心肌细胞后的转染效率及Rad基因的表达情况

来源 :第二十四届长城国际心脏病学会议、亚太心脏大会暨国际心血管病预防与康复会议(The 24th Great Wall Int | 被引量 : 0次 | 上传用户：thriving_hehe

【摘要】

：

目的:研究外源性Rad基因重组腺病毒在体外转染乳鼠心肌细胞后的转染效率及Rad基因的表达情况.方法:构建Rad腺病毒载体,以不同的MOI值转染体外培养的原代乳鼠心肌细胞,通过荧光显微镜观察转染后荧光强度及转染效果,流式细胞仪检测转染率,采用RT-PCR、Western blot检测Rad基因表达情况.

【作者】

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耿召华黄岚

【机构】

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第三军医大学新桥医院全军心血管病研究所

【出处】

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第二十四届长城国际心脏病学会议、亚太心脏大会暨国际心血管病预防与康复会议(The 24th Great Wall Int

【发表日期】

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2013年2期

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研究外源性Rad基因重组腺病毒在体外转染乳鼠心肌细胞后的转染效率及Rad基因的表达情况

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