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Photodynamic therapy(PDT)is currently an advanced optical technology in medical applications.However,the application of PDT is limited by the detection of photosensitizers used in PDT.This work focuses on the application of fluorescence spectroscopy and fluorescence imaging in the detection of an effective photosenzitizer,hematoporphyrin monomethyl ether(HMME).The optical properties of HMME were measured and analyzed based on absorption and fluorescence spectra.