A novel amine monomer trimesoylamidoamine (TMAAM) was synthesized from trimesoyl chloride (TMC) and 1,3-diamino-2-propanol (DAP) and then be used to fabricate the new polyamide thin-film composite (TFC) reverse osmosis (RO) membranes.The membrane fabrication method had an important influence on the membrane structure and performance.The novel DAP/TMAAM-TMC membrane was prepared via conventional interfacial polymerization method with the mixed amine aqueous phase of DAP and TMAAM, while the DAP-TMAAM-TMC membrane was fabricated through two-step amine immersion method.The chemical structure of the resulted membranes were analyzed by attenuated total reflectance infrared(ATR-FTIR) and X-ray photoelectron spectroscopy (XPS), and their morphologies were observed by scanning electron microscopy (SEM) and atomic force microscopy(AFM).The separation performance of the membranes was evaluated by permeate experiment, and their antifouling and chlorine tolerant properties were tested by dynamic fouling experiment and exposure experiment to active chlorine.The results show that the DAP-TMAAM-TMC presents much smoother surface, relative better antifouling and chlorine tolerant properties than the conventional MPD-TMC membrane and DAP/TMAAM-TMC membrane.