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We describe the fabrication of a novel electrochemical deoxyribonucleic acid (DNA) biosensor based on electrochemical reduced graphene oxide (ERGO) and peptide nucleic acid (PNA)-DNA hybridization.Primarily, the ERGO-modified electrode was achieved by one-step electrodeposition technique on the glassy carbon electrode (GCE) through cyclic voltammetric (CV)reduction of a graphene oxide colloidal solution.