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Koi herpesvirus disease(KHVD)is associated with high mortality in both common carp and koi carp(Cyprinus carpioL.)worldwide.The indirect detection of fish viruses based on the identification of antibodies has emerged as a practical and reliable means of diagnosis.Thus,it is important to create monoclonal antibodies(MAb)against carp IgM.By using hybridoma-monoclonal antibody technology,one hybridoma cell line secreting MAbs against the IgM from carp was established.By western blot analysis,the secreted MAb from cell lines A5-E10 recognized the heavy chain of carp IgM,and did not react with immunoglobulins from three different fish species: grass carp(Ctenopharhyngodon idella),tilapia(Oreochromis mossambicus)and Mandarin fish(Siniperca chuatsi).The result demonstrated that MAb was highly specific and sensitive to the Ig of carp,and can be used for monitoring the immunity level of carp,for example,the indirect KHV diagnosis by antibody ELISA.An indirect ELISA method was established using the MAb A5-E10 to detect antibodies in koi sera for the detection KHV antibodies in koi sera.A total number of 200 serum samples from koi were collected from three farms and tested by antibody ELISA.The final results showed that 147(73.5#)samples were confirmed as KHV antibody negative and 53(26.5#)were definitely positive,bearing antibodies against KHV.