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The deregulation of microRNA (miRNA) is frequently associated with avariety of cancers,including hepatocellular carcinoma (HCC).In this study, we identified 10 up-regulated miRNAs (miR-217, miR-518b,miR-517c, miR-520g, miR-519a, miR-522, miR-518e, miR-525-3p, miR-512-3p, and miR-518a-3p) and 10down-regulated miRNAs (miR-138, miR-214, miR-214#, miR-27a#, miR-199a-5p, miR-433, miR-511, miR-592,miR-483-5p andmiR-483-3p) by Taqman miRNAs array and real time RT-PCR confirmation.Furthermore,we investigated the expression and possible role of miR-138 in HCC.Quantitative PCR results showed that miR-138was down-regulated in 76.2%(14/18) of HCC tissues compared with adjacent nontumor tissues.Overexpression of miR-138 in HCC cell lines inhibited tumor cell growth and colony formation by induction of cell arrest.Using TargetScan predictions, CCND3 was defined as a potential direct target of miR-138.Furthermore, CCND3 protein expression was observed to be negatively correlated with miR-138 expression in HCC tissues.