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Introduction: The effects of the ovary hormones on hepatic FMO3 expression have been detected in mice,suggesting potential relationship is existed between FMO3 mRNA expression and hormones.Here, we determined wheather the FMO3 gene played an important role in regulating secretion of hormones on the process of goose egg-laying.Materials and Methods: Tissue samples from the ovary during pre-laying (22 weeks of age), peak-laying (45 weeks of age) and ceased-laying (64 weeks of age) period (each five female individuals) were collected from Sichuan white goose and Xupu goose.