论文部分内容阅读
Introduction Circadian clock, an internal timekeeping mechanism, allows a variety of organisms to adapt their physiological and behavioral processes to a changing external environment.The metabolism and behavior of mammals such as mice show a day-night rhythm.The circadian clock can be entrained by many cues.H2S is an endogenous gas signaling molecule and closely relates to energy metabolism.However, the role of H2S in the regulation of circadian rhythm is largely unknown.Methods We utilized an intraventricular infusion approach to directly deliver NaHS (H2S donor) or amiono-oxyacetate (AOAA), an inhibitor of cystathionine-β-synthase (CBS),to the leteral cerebroventricles of C57 mice.The locomotor rhythm of mice was recorded by the VitalView wheelrunning system.Immunohistochemistry and real-time quantitative PCR displayed the expression of CBS and clock genes.Results Infusion of H2S donor or CBS inhibitor both exerted a phase delay of locomotor rhythm in the C57mice.The phase shift induced by CBS inhibitor was similar to light-induced phase shift, but they were independent and showed different delayed time.H2S promoted the expression of sirtl and decreased the expression of per2.The protein expression of CBS in the hypothalamic suprachiasmatic nuclei (SCN) of mice SCN exhibited a circadian rhythm.Conclusion H2S contributed to the phase-shift of free-running mice, which may be by the pathway of affecting the rhythmic energy metabolism.