Nowadays,interest in using biodegradable materials to substitute for synthetic plastics in food packaging has increased because of the consumers awareness of environmental damage caused by non-biodegradable packaging.Whey protein can produce transparent,flexible,favourable smell edible films.Addition of glutathione transglutaminase (TG) can help proteins form both inter-and intra-molecular isopeptide bonds by cross-linking of the amino acid residues of protein bound glutamine and lysine,which can greatly improve the barrier ability of whey protein film.The molecular weight of whey protein after TG interaction was assayed by HPLC.The denatured temperature of whey protein was determined by DSC.