Counter-current chromatography (CCC),as a unique and important tool for separation and purification of natural products,possesses a peculiar characteristic that is support-free liquid-liquid chromatography,thus it eliminates such complications occurred in common column chromatography as sample loss,tailing of solute peaks and contamination.That is why counter-current chromatography is gaining more and more popularity in modern separation of natural products despite of it’s a little lower resolution than gas chromatography (GC) and high-performance liquid chromatography (HPLC) or other high-resolution column chromatography.Nowadays,resolution,separation time and sample loading capability is what hinder CCC development most seriously,although the CCC methods and related technologies have been improved rapidly due to worldwide researchers constant effort.Natural products,as a very rich source of biologically active compound for new drug,chemical molecular,protein and gene discovery,are needed to be analysis and prepared more quickly.So high-throughput methods and technologies are pivotal for the CCC advancement.This work will provide a 2-dimension (2D) counter-current chromatography design which is illustrated by figure 1.We can realize the shift from single parallel separation to 2D CCC separation by switching the valve II (blue one in the figure) and valve III (green one in the figure) only.So we can add more loading sample due to the increase of the column volume,which can also improve separation performance.Moreover,this design can help us save labor work of assembling these instruments just by switching these two valves.Figure1.the design program for multi-dimension CCC If valve II is in state 1-2 while valve III is in state 1-2,the three columns are on parallel separation mode; if valve II is in state 1-2 while valve III is in state 1-6,column A and column C are connected; if valve II is in state 1-6 while valve Ⅲ is in state 1-6,column A and column B are connected; if valve Ⅱ is in state 1-6 while valve III is in state 1-2,column C is parallel with column A-B.