【摘 要】
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Background: Xuesaitong soft capsule (XST) which consists of panax notoginseng saponin (PNS) has been used to treat ischemic cerebrovascular diseases in China.The therapeutic mechanism of XST has not b
【机 构】
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中西医结合,广安门医院心血管科,北京中医药大学,北京市朝阳区北三环东路11号,100029
【出 处】
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2015全国中医药博士生创新发展论坛
论文部分内容阅读
Background: Xuesaitong soft capsule (XST) which consists of panax notoginseng saponin (PNS) has been used to treat ischemic cerebrovascular diseases in China.The therapeutic mechanism of XST has not been elucidated yet from prospective of genomics and bioinformatics.Methods: A transcriptome analysis was performed to review series concerning middle cerebral artery occlusion (MCAO) rat model and XST intervention after MCAO from Gene Expression Omnibus (GEO) database.Differential expressed genes (DEGs) were compared between blank group and model group,model group and XST group.Functional enrichment and pathway analysis were performed.Protein-Protein interaction network was constructed.The overlapping genes from two PPI networks were screened out and profound analysis was performed.Results: Two series including 22 samples were obtained.870 DEGs were identified between blank group and model group,and 1189 DEGs were identified between model group and XST group.GO terms and KEGG pathways of MCAO and XST intervention were significantly enriched.PPI networks were constructed to demonstrate the gene-gene interactions.The overlapping genes from two DEGs sets were highlighted.Antxr2,Fhl3,Prep,Tyrobp,Tp53,Cdk2,SMAD4 and Stat1 were the pivotal genes and possible action sites of XST therapeutic mechanisms.Conclusion: MCAO is a pathological process with multiple aspects.XST could achieve therapeutic effects on ischemic cerebrovascular diseases via specific genes.
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