【摘 要】
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Objective To investigate the change patterns of Histone 3 lysine 9 acetylation (H3K9ac) and the expression profile of histone deacetylase 1(HDAC1), HDAC3, HDAC4 and HDAC6 in zebrafish development.Meth
【机 构】
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Department of Molecular Biology, Hebei Medical University, Shijiazhuang, China
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Objective To investigate the change patterns of Histone 3 lysine 9 acetylation (H3K9ac) and the expression profile of histone deacetylase 1(HDAC1), HDAC3, HDAC4 and HDAC6 in zebrafish development.Methods In the study, the zebrafish embryo at the developmental stages of 24h, 48h and 72h post-fertilization were collected, and the content of H3K9ac and the expression profile of HDAC1, HDAC3, HDAC4 and HDAC6, as well as the content of HDAC3, was evaluated.Results The content of H3K9ac significantly decreased in the 48h group, indicating a role of H3K9-deacetylation in the middle developmental stages of zebrafish.The expression profile of HDAC1, HDAC3, HDAC4 and HDAC6 changed along with the zebrafish development, and the changes of HDAC3 were consistent with that of H3K9-deacetylation.Furthermore, the content of HDAC3 confirmed its expression changes, manifesting that HDAC3 may play an important role in H3K9-deacetylation at the 48h group.Conclusion The change patterns of H3K9ac and HDACs in zebrafish development were revealed, and H3K9-deacetylation and HDAC3 might participate in the middle developmental stages of zebrafish.
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