In this study, for the first time, we report on a facile method for the synthesis and immobilization of AuNPs on m-aminophenol based resin (MAPR) microspheres via a simple reduction route.The AuNPs decorated MAPR (AuNPs/MAPR) microspheres with a lot of activity groups (amino and hydroxy groups) can not only act as suitable immobilization carriers for antibody (EGFR antibody), but also play an important role in facilitating the electron transfer.