【摘 要】
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To establish molecular monitoring for the Gambierdiscus community in tropical sea, we analyzed the restriction fragment length polymorphism of large subunit ribosomal rDNA sequences of D1-D2 region fr
【机 构】
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Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, Xiamen University, X
【出 处】
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第六届全国微生物资源学术暨国家微生物资源平台运行服务研讨会
论文部分内容阅读
To establish molecular monitoring for the Gambierdiscus community in tropical sea, we analyzed the restriction fragment length polymorphism of large subunit ribosomal rDNA sequences of D1-D2 region from ten Gambierdiscus species and several environmental samples.The goals of the present study were to devise restriction fragment length polymorphism based assays for distinguishing Gambierdiscus spp.Computer assist examination of the sequences allowed us to identify Aci I that were predicted to reveal species large subunit rDNA heterogeneities.Some stains identified by sequencing were revealed independently and repeatedly by RFLP analysis of PCR amplified product.The RFLP texts described here are applicable at a fraction of the time, cost and labor required for sequencing.
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