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目的比较红花当归单煎共煎液中有效成分羟基红花黄色素A和阿魏酸含量的变化,初步探索2味中药配伍的变化规律。方法采用RP-HPLC,色谱柱为Shim-pack VP-ODS C18(4.6 mm×150 mm,5μm),流动相为甲醇-0.02 mol.L-1磷酸二氢钾缓冲液(磷酸调节pH至3.5),采用梯度洗脱进行分析;检测波长为230 nm,流速为1.0 mL.min-1。结果羟基红花黄色素A回归方程Y=22 821ρ-4 105.1,r=0.999 9,在2.5~50 mg.L-1内线性相关良好,平均加样回收率为100.1%,RSD为2.2%;阿魏酸回归方程Y=70 219ρ-2 573.9,r=0.999 9,在0.5~10 mg.L-1内线性相关良好,平均加样回收率为101.4%,RSD为3.0%。结论红花当归共煎液中羟基红花黄色素A和阿魏酸含量比各单煎液中含量高。共煎更有助于有效成分的提取。本方法简便、快速、准确,适用于同时测定羟基红花黄色素A和阿魏酸的含量。
Objective To compare the changes of the contents of hydroxysafflor yellow A and ferulic acid in active ingredient of Safflower Angelica Decoction Decoction, and to explore the variation regularity of compatibility of 2 herbs. Methods The mobile phase consisted of methanol-0.02 mol·L-1 potassium dihydrogen phosphate buffer (phosphoric acid adjusted to pH 3.5) using Shim-pack VP-ODS C18 (4.6 mm × 150 mm, 5 μm) , Using gradient elution analysis; detection wavelength of 230 nm, the flow rate of 1.0 mL.min-1. Results The regression equation of hydroxysafflor yellow A was linear in the range of 2.5 ~ 50 mg.L-1 with the average recovery of 100.1% and the RSD of 2.2% with Y = 22 821 p-4 105.1 and r = 0.999 9. The regression equation of ferulic acid Y = 70 219ρ-2 573.9, r = 0.999 9, good linear correlation within 0.5 ~ 10 mg.L-1, the average recovery was 101.4% with RSD 3.0%. Conclusion The contents of hydroxysafflor yellow A and ferulic acid in safflower Angelica decoction decoction are higher than those in decocted decoction. A total of more helpful to the extraction of active ingredients. The method is simple, rapid and accurate and is suitable for the simultaneous determination of hydroxysafflor yellow A and ferulic acid.