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目的:观察麦门冬汤对BAL B/C硬皮病小鼠外周血CD4+、CD8+T细胞及腹腔巨噬细胞活力的影响。方法:选8周龄60只BALB/C小鼠,实验前剃去小鼠背部中央区被毛,并随机分为5组,每组12只,分别为正常对照组(正常组)、模型对照组(模型组)、麦门冬汤大剂量组、麦门冬汤中剂量组、麦门冬汤小剂量组。除正常组外,其他组用博莱霉素(BL M)制作硬皮病模型。使用麦门冬汤大、中、小剂量治疗3周。眼球采血,用流式细胞仪采用单平台操作法检测CD4+、CD8+T细胞。进行腹腔灌洗并收集灌洗液,用预冷RPMI-1640培养液洗涤细胞3次,每次4℃1500 rpm离心10 min,去上清液,再用预冷的适量RPM I-1640培养液悬浮细胞。用移液器取100 LL的细胞悬液,加100 LL的胎盼蓝与800 LL的RPM I-1640放入1.5mL的离心管中染色,计算腹腔巨噬细胞活力。结果:麦门冬汤小剂量组CD4+T细胞的含量高于模型组(P<0.01);大、中剂量组CD8+T细胞含量高于模型组(分别为P<0.01和P<0.05)。中、小剂量组腹腔巨噬细胞活力均高于模型组(P<0.01)。结论:麦门冬汤能提高CD4+、CD8+T细胞水平,提高模型小鼠腹腔巨噬细胞的活力。这可能是麦门冬汤对SSc模型小鼠发挥作用的机制之一。
Objective: To observe the effect of Maimen Dong Decoction on the activity of CD4 +, CD8 + T cells and peritoneal macrophages in BALB / c scleroderma mice. Methods: Sixty BALB / C mice (8 weeks old) were selected and shaved off the central area of the back of the mice before the experiment. They were randomly divided into 5 groups (12 in each group), which were normal control group (normal group), model control Group (model group), high dose group of Mai Men Dong Tang, middle dose group of Mai Men Dong Tang, small dose group of Mai Men Dong Tang. In addition to the normal group, scleroderma models were made with bleomycin (BL M) in other groups. Use Mai Men Dong Tang large, medium and small doses of treatment for 3 weeks. Eye blood collection, using flow cytometry single platform method to detect CD4 +, CD8 + T cells. The peritoneal lavage was performed and the lavage fluid was collected. The cells were washed three times with pre-cooled RPMI-1640 medium and centrifuged at 1500 rpm for 4 hours at 10 min. The supernatant was then centrifuged and the precooled RPM I-1640 medium Suspension cells. Pipette 100 L of cell suspension, add 100 LL of fetal trypan blue and 800 LL of RPM I-1640 into 1.5 mL centrifuge tube staining to calculate peritoneal macrophage viability. Results: The content of CD4 + T cells in the small dose group of Meimen Dong decoction was higher than that in the model group (P <0.01). The contents of CD8 + T cells in the large and medium dose groups were higher than those in the model group (P <0.01 and P <0.05, . The activity of peritoneal macrophages in medium and low dose groups were higher than those in model group (P <0.01). Conclusion: Mai-meng-dong decoction can increase the level of CD4 +, CD8 + T cells and increase the peritoneal macrophage viability in model mice. This may be one of the mechanisms that MaiMenDong Decoction can play a role in SSc model mice.