We show that an asymmetrical AC electrical fields (AACEf) can induce the same anatomical responses and preferred symmetry from Sympathetic Neurons in vitro as does in DC electrical fields (DCEf).Briefly, the imposition of the AACEf induces a rapid retraction of neurites that prior to exposure were parallel/tangential to the lines of force.The latter was determined to be < 15 ° from perpendicular to the long axis of the experimental chamber (or alternately, perpendicular to the lines of force of the imposed electrical fields.These neuritis retract rapidly beginning < 5 min after exposure.Those that partially or completely retracted began to regrow, reextending to a preferred orientation perpendicular to the lines of force.Neurites that were already oriented in the Perpendicular plane did not retractbut were stimulated to extend in that direction.Neurons were monitored by time lapse photomicroscopy;the lengths of new growth, and the rates of extension and retraction of processes were compared quantitatively.Symetrical AC electrical fields (SACEf) did not effect the neurons over the 5-6 hour observation period.AACEf group 1 was produced by a waveform in which the magnitude of both polarities of the pulse were identical, but the duration of the "negative" component was ~ 5 times shorter than the positive component.This produced the most marked responses relative to another tested waveform (AACEf group 2).In this stimulation paradigm, the reversed negative polarity was about1/2that of the positive component, while the duration of each component was identical to AACEf group 1.While the overall anatomy and preferred orientation of sympathetic neurons was identical to DC applications, the rates of regrowth, retraction, and the lengths of newly extended neurites was statistically significantly reduced in the AC applications.This research is an important bridge to a noninvasive means to modify nerve growth and organization by magnetic inductive coupling at distance, which will greatly reduce the pain and risk of surgery for nerve regeneration.