【摘 要】
：
The gone of diphosphomevalonate decarboxylase was cloned successfully from Euphorbia helioscopia L.,named as EhMDC (accession number: KP995936).The full length cDNA of EhMDC was 1653 bp,and it contain
【机 构】
：
Key Laboratory of Resource Biology and Biotechnology in Western China, (Northwest University) Minist
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The gone of diphosphomevalonate decarboxylase was cloned successfully from Euphorbia helioscopia L.,named as EhMDC (accession number: KP995936).The full length cDNA of EhMDC was 1653 bp,and it contained an open reading frame of 1245 bp,a 5untranslated region of 184 bp and a Y untranslated region of 224 bp.Diphosphomevalonate decarboxylase contained 415 amino acids.Bioinformatic analysis showed that it was a hydrophilic protein with predicted molecular weight of 45.971 kDa and theoretical pI of 5.77.It was located in the cytoplasm and belonged to the membership of GHMP kinases superfamily.It included predicted phosphorylation sites of 19 amino acids,and it has not transmembrane domain and signal peptide.
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