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Interleukin-21(IL-21) was recently discovered using a functional cloning approach based on expression of its receptor,has been found it influences proliferation of T and B cells and cytolytic activity of natural killer cells,but little is known about its distribution in the thymus of African ostrich chicks.In the present study,the distribution,morphological characteristics,and developmental changes of IL-21-producing cells in the thymus of African ostrich chicks were investigated using immunohistochemistry.