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采用季戊四醇/辛酸亚锡引发体系通过开环聚合制备了四臂星形聚己内酯。将丙烯酰氯和肝素分别引入到四臂聚己内酯的端基上,以赋予材料可交联和抗凝性能。采用GPC、红外、核磁等表征了四臂星形聚己内酯的结构与性能。静态接触角和吸水率测试结果显示接枝肝素后聚己内酯的亲水性明显增强。采用紫外定量表征了肝素化聚己内酯表面的肝素含量,体外凝血时间的明显延长说明了肝素化后材料具有了抗凝性能。在添加适量光引发剂的情况下用紫外光对含有丙烯酰氯基团的肝素化聚己内酯进行了原位交联,力学测试表明光照后材料的拉伸强度明显提高。最后对材料通过静电纺丝技术成型管状支架作了初步研究,表明本文合成的抗凝可交联聚己内酯材料在血管组织工程领域具有良好的应用前景。
Four-arm star polycaprolactone was prepared by ring-opening polymerization using pentaerythritol / stannous octoate initiator system. Acryloyl chloride and heparin were introduced into the four-terminal polycaprolactone end groups to give the material cross-linking and anticoagulant properties. The structure and properties of four-arm star polycaprolactone were characterized by GPC, IR, NMR and so on. Static contact angle and water absorption test results show that the hydrophilicity of polycaprolactone after grafted heparin is obviously enhanced. The heparin content of heparinized polycaprolactone was characterized by UV quantification, and the prolongation of coagulation time in vitro indicated that the heparinized material has anticoagulant properties. In situ crosslinking of heparinized poly (caprolactone) containing acryloyl chloride with UV light was carried out with the addition of a suitable amount of photoinitiator. The mechanical tests showed that the tensile strength of the material increased significantly after irradiation. Finally, a preliminary study on the material forming of the tubular scaffold by electrospinning technology shows that the anticoagulant crosslinkable polycaprolactone material synthesized in the present invention has a good application prospect in the field of vascular tissue engineering.