Polycyclic aromatic hydrocarbons(PAHs)were considered as a source of carcinogenicity in mainstream cigarette smoke(MSS).Accurate quantification of these components was necessary for assessing public health risk.In our study,a solid-phase extraction(SPE)method using graphene oxide(GO)bound silica as adsorbent for purification of 14 PAHs in MSS was developed.During SPE process,large matrices interferences of MSS were adsorbed on SPE column.The result of FTIR spectra demonstrated that these matrices interferences were adsorbed on GO mainly through O H and C O groups.The concentrations of PAHs in MSS extract were determined by gas chromatography–mass spectrometry(GC–MS).The limit of detection(LOD)and limit of quantification(LOQ)of the developed method for 14 PAHs ranged from 0.05 to 0.36 ng/cig and 0.17 to 1.19 ng/cig,respectively.The accuracy of the measurement of 14 PAHs was from 73 to 116%.The relative standard deviations of intra-and inter-day analysis were less than 7.8%and 13.9%,respectively.Moreover,the developed method was successfully applied for analysis of real cigarette containing 1R5F reference cigarette and 12 top-selling commercial cigarettes in China.