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Objective: Our aim is to examine the potential of poly(D,L-lactide-co-glycolide acid) (PLGA) nanoparticles (NPs) for carrying single or compound drugs traversing the round window membrane (RWM) after round window (RW) administration to guinea pigs.Methods: Coumarin-6 loaded PLGA NPs were prepared by the emulsion/solvent evaporation method.