Colletotrichum Cda.is one of worldwide plant pathogens causing anthracnose before or after maturity in lots of crops, which had led great economical lose.The classification and identification of plant pathogenic Colletotrichum species has been confusing, difficult to accurately identify pathogen species, brought disorder to study such diseases and affects the control effect.Therefore, it is very necessary to accurate identification and rapid detection studies of Colletotrichum.In 2012 ~ 2014, we collected about 1000 samples from some areas of Henan, Guangdong, Guangxi, Beijing, Yunnan, Hunan and Hainan Provinces in China.The hosts plants included 36 families, 59 genera;85 strains obtained by single spore isolation and tissue separation.45 typical strains were divided into four species complex groups based on the morphological characteristics.We combined ITS, GAPDH, ACT, TUB2, CAL, CHS-1 gene with the related type strains published or representative and reliable strain sequence to establish the multi-gene phylogenetic trees.Then, we had identified 21 Colletotrichum species by morphological characteristics and multilocus molecular sequence analyses by compared them with neighboring species on NJ phylogenetic tree constructed by multi-gene combining including, including six new species, Colletotrichum tamarindum Meng Zhang & C.D.Zhang, Colletotrichum sanderianum Meng Zhang & C.D.Zhang, Colletotrichum hostum Meng Zhang & C.D.Zhang, Colletotrichum racemosum Meng Zhang & C.D.Zhang, Colletotrichum viticola Meng Zhang & C.D.Zhang and Colletotrichum pandanicola Meng Zhang & C.D.Zhang.In addition, 2 species, Colletotrichum fioriniae J.H.Simmonds, Colletotrichum scovillei Damm, P.F.Cannon & Crous are new records in China.