【摘 要】
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Objective To extract exosomes from breast cancer cell line MDA-MB-231 and to analyze their origin and molecular biological characteristics.Methods Exosomes were isolated from breast cancer cells by mu
【机 构】
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School of biomedical engineering,Shanghai Jiao Tong University,Shanghai 200230,China;Shanghai Center
【出 处】
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中华医学会第十四次全国妇科肿瘤学术会议
论文部分内容阅读
Objective To extract exosomes from breast cancer cell line MDA-MB-231 and to analyze their origin and molecular biological characteristics.Methods Exosomes were isolated from breast cancer cells by multi-step differential centrifugation and were further examined with atomic force microscope.The expression of marker gene miR-21 was measured by RT-PCR, and the expression of marker protein Flotillin-1 was validated by Western blot.
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当今妇科手术发展围手术期定义围手术期定义■围手术期(periopemtive period)见于20世纪70年代■1981年第26版Dolands医学词典解释:围手术期是指"从病人因需手术治疗而住院起到出院时为止的期限"■ 1988年11月中国人民解放军第一届普外科围手术期学术讨论会:围手术期是指从确定手术治疗时起,至与这次手术有关的治疗基本结束为止的一段时间
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Object Drug resistance remains a major clinical obstacle to successful treatment in ovarian cancer patients, and the evidence of microRNAs involvement in drug resistance has been emerging recently.In
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